Electrophoretic separation of nervous system proteins on exponential gradient polyacrylamide gels
β Scribed by P. T. Kelly; M. W. Luttges
- Book ID
- 111167842
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- English
- Weight
- 380 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0022-3042
No coin nor oath required. For personal study only.
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An electrophoretic system for separating, with high resolution, peptides 25-250 residues in length is described. The peptides are stacked by discontinuous electrophoresis to form very sharp bands at the origin. They are then separated on a matrix of 20% polyacrylamide, 8 M urea, and 0.1% dodecyl sul
A nondenaturing gradient polyacrylamide gel electrophoresis method is described for the resolution of membrane proteins. Bovine heart inner mitochondrial membranes were solubilized in Triton X-100 and individual complexes were identified by staining for activity and protein. Succinate dehydrogenase