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A novel polymorphism (6376 G/T) in intron 7 of the human protein C gene

✍ Scribed by José Manuel Soria; Marta Morell; Xavier Estivill; Núria Sala

Publisher
Springer
Year
1995
Tongue
English
Weight
201 KB
Volume
96
Category
Article
ISSN
0340-6717

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## Detection conditions: Forward primer: 5´-ATT ACG TCC CAG CGT CTG AG-3Ŕ everse primer: 5´-ATA AAT CAG CTT GCT TAG TGC C-33 5 cycles; denaturation at 94°C (20 s), annealing at 60°C (20 s), extension at 72°C (20 s), final extension 72°C (10 min) 10% PAA gels (49:1, acrylamide:bisacrylamide), 0.5XTB