A novel method for measurement of the specific radioactivity of γ-32P ATP
✍ Scribed by R.H. Hammerstedt; W.G. Niehaus Jr.
- Book ID
- 107711663
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 483 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0003-2697
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📜 SIMILAR VOLUMES
A new, rapid method for the measurement of [y3\*P]ATP specific radioactivity in tissue extracts in the presence of other 32P-containing compounds is described. The deproteinized extract is incubated with phosphorylase b and phosphorylase kinase, and the incorporation of 3zP into protein from [Y-~\*P
The specific radioactivity of a choline phospholipid has been determined by a double-isotope method. Purified phospholipid was hydrolyzed to release labeled choline, and choline kinase was employed to label the choline with 32P from [-Y-~\*P]ATP. The double-labeled phosphorylcholine was purified by