An easy and rapid method for the measurement of [γ-32P]ATP specific radioactivity in tissue extracts obtained from in vitro rat heart preparations labeled with 32Pi

A rapid method for the measurement of [γ

A rapid method for the measurement of [γ-32P]ATP specific radioactivity in tissue extracts and its application to the study of 32Pi uptake in perfused rat heart

✍ Paul J. England; Donal A. Walsh 📂 Article 📅 1976 🏛 Elsevier Science 🌐 English ⚖ 438 KB

A new, rapid method for the measurement of [y3\*P]ATP specific radioactivity in tissue extracts in the presence of other 32P-containing compounds is described. The deproteinized extract is incubated with phosphorylase b and phosphorylase kinase, and the incorporation of 3zP into protein from [Y-~\*P