A method for measurement of the specific radioactivity of the choline moiety of choline phospholipids

✍ Scribed by William A. Rowan Jr.; Roy H. Hammerstedt

• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 651 KB
• Volume: 81
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90611-x

✦ Synopsis

The specific radioactivity of a choline phospholipid has been determined by a double-isotope method. Purified phospholipid was hydrolyzed to release labeled choline, and choline kinase was employed to label the choline with 32P from [-Y-~*P]ATP. The double-labeled phosphorylcholine was purified by ion-exchange chromatography on QAE-Sephadex, and the specific radioactivity of the choline was calculated from the isotope ratio. The method is sensitive, requiring only 5 nmol of choline with a specific radioactivity of I ~Cii~mol. and the chromatographic isolation of phosphorylcholine is simple and reproducible.