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A radiometric method for the determination of choline acetylase activity based on thin-layer chromatography

✍ Scribed by Zoran Grubić; Tomazˇ Kiauta; Miro Brzin

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 285 KB
Volume: 74
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90216-5

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✦ Synopsis

A rapid thin-layer chromatographic method on Gelman I.T.L.C. type SA plates for the determination of choline acetylase activity has been developed. The mobile phase consists of sodium tetraphenylboron dissolved in acetonitrile. The Rt values for acetyl coenzyme A and acetate are about 0.1, while that for acetylcholine is about 0.9. The method compares favorably with other radiometric methods as far as recovery, separation, and simplicity are concerned. MATERIALS AND METHODS Chemicals. [ lJ4C]Acetylcholine chloride (sp act 11.8 mCi/mmol), [1-14C]acetate (sp act 58 mCi/mmol), and [1-14C]acetyl coenzyme

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