𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A novel method for efficient expression cloning of fungal enzyme genes

✍ Scribed by Dalbøge, H. ;Heldt-Hansen, H. P.

Publisher
Springer
Year
1994
Tongue
English
Weight
957 KB
Volume
243
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

A versatile shuttle cosmid vector for th
A versatile shuttle cosmid vector for the efficient construction of genomic libraries and for the cloning of fungal genes
✍ Heinz D. Osiewacz 📂 Article 📅 1994 🏛 Springer-Verlag 🌐 English ⚖ 404 KB

A shuttle cosmid vector, pANsCos1, has been constructed for Escherichia coli and filamentous fungi. This vector contains two cos sequences separated by a single XbaI restriction site. pANsCos1 allows the efficient construction of representative genomic libraries from as little as 15-20 micrograms of

Molecular cloning of genes expressed spe
Molecular cloning of genes expressed specifically during induction of cell wall degrading enzymes fromSclerotinia sclerotiorumand preliminary identification of a fungal beta-galactosidase encoding gene by expression inEscherichia coli
✍ Gabriel Waksman 📂 Article 📅 1988 🏛 Springer-Verlag 🌐 English ⚖ 278 KB

Cell wall degrading enzymes and oxalic acid have been associated with diseases caused by Sclerotinia sclerotiorum. First attempts to clone S. sclerotiorum cell wall degrading enzymes are presented here. A gene library has been constructed in the lambda insertion vector lambda 1149. The library has b

Efficient recovery of environmental DNA
Efficient recovery of environmental DNA for expression cloning by indirect extraction methods
✍ Esther M Gabor; Erik J de Vries; Dick B Janssen 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 256 KB

Using direct and cell extraction-based (indirect) isolation methods, DNA was obtained from environmental samples with largely differing characteristics (loam soil, sand soil, sediment, activated sludge, and compost) and evaluated with respect to the comprised bacterial diversity and its suitability

Cloning and heterologous expression of a
Cloning and heterologous expression of a novel arylmalonate decarboxylase gene fromAlcaligenes bronchisepticusKU 1201
✍ Kenji Miyamoto; Hiromichi Ohta 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 717 KB

We have cloned and sequenced a DNA fragment that encodes the arylmalonate decarboxylase (AM-Dase) gene from Alcaligenes bronchisepticus KU 1201. The AMDase gene consists of an open reading frame of 720 nucleotides, which specifies a 240-amino-acid protein of relative molecular mass (Mr) 24734. The