A novel binding assay for phospholipase A2
β Scribed by Susan H. Peers; Roger D. Taylor; Roderick J. Flower
- Book ID
- 115779993
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 553 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0006-2952
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π SIMILAR VOLUMES
A rapid and continuous method for measuring phospholipase A2 activity using electron spin resonance spectroscopy and a spin-labeled phospholipid as a substrate has been developed. The substrate, 1-palmitoyl-2-(4-doxylpentanoyl)glycerophosphocholine, gives rise principally to a broad ESR line in aque
A fluorescence assay for quantitation of human Type II Phospholipase \(A_{2}\) activity is described. Hydrolysis of 1-Acyl-2-(N-4-nitrobenzo-2-oxo-1,3-diazole)aminododecanoyl Phosphatidylethanolamine is accompanied by an increase in fluorescence intensity that is linearly proportional to enzyme acti