A rapid and continuous method for measuring phospholipase A2 activity using electron spin resonance spectroscopy and a spin-labeled phospholipid as a substrate has been developed. The substrate, 1-palmitoyl-2-(4-doxylpentanoyl)glycerophosphocholine, gives rise principally to a broad ESR line in aque
Polarographic assay for phospholipase A2
โ Scribed by Paul H. Gale; Robert W. Egan
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 375 KB
- Volume
- 104
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A new assay for phospholipase C activity that uses alkaline phosphatase to convert phosphorylchohne to inorganic phosphate is described. The determination of inorganic phosphate is performed in the presence of phosphatidylcholine and protein after the addition of sodium dodecyl sulfate. Phospholipas
A continuous, coupled polarographic assay, which couples trehalose hydrolysis to OZ consumption using glucose oxidase (EC 1.1.3.4) and catalase (EC 1.11.1.6) as ancillary enzymes has been developed for the measurement of trehalase (o-a'-trehalose 1-D-glucohydrolase, EC 3.2.1.28) activity. With this