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A non-phenol–chloroform extraction of double-stranded RNA from plant and fungal tissues

✍ Scribed by Alitukiriza Balijja; Anders Kvarnheden; Tullio Turchetti


Book ID
113767849
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
700 KB
Volume
152
Category
Article
ISSN
0166-0934

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A procedure for extracting DNA, RNA, and protein from an L1210 leukemia cell sample is described. Sodium deoxycholate and 154 mM NaCl in the presence of phenol extracts cytoplasmic RNA. Sodium deoxycholate, paminosalicylate, and 1 M NaCl in the presence of phenol extracts the DNA. The DNA is then fr