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A new method for extraction of double-stranded RNA from plants

✍ Scribed by Ioannis E. Tzanetakis; Robert R. Martin


Book ID
113767757
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
414 KB
Volume
149
Category
Article
ISSN
0166-0934

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A simple method for separation of ssRNA from dsRNA on hydroxyapatite is described. The following procedure can be usefully applied for RNA fractionation: (i) Elution of column with 0.1 M phosphate at 60" removes nueleotidrs and a sm:ilI amount of ssRNA. (ii) Elution with 0.1 31 phosphate at 90" remo