𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A nitrocellulose filter binding assay for ribonucleotide reductase

✍ Scribed by Karin Söderman; Peter Reichard

Book ID
107714284
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
404 KB
Volume
152
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

An ultrafiltration assay for nucleotide
An ultrafiltration assay for nucleotide binding to ribonucleotide reductase
✍ Mats Ormö; Britt-Marie Sjöberg 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 459 KB

Direct partition through ultrafiltration was applied to develop a method for the study of nucleotide binding to ribonucleotide reductase from Escherichia coli. The assay involved a 0.5- to 1-min centrifugation step where bound and unbound nucleotides are separated over an ultrafiltration membrane. N

Is Nitrocellulose Filter Binding Really
Is Nitrocellulose Filter Binding Really a Universal Assay for Protein–DNA Interactions?
✍ Stefan Oehler; Regina Alex; Andrew Barker 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 152 KB

The ability to bind to nitrocellulose is commonly accepted as being a universal property of proteins and has been widely used in many different fields of study. This property was first exploited in the study of DNAbinding proteins 30 years ago, in studies involving DNA binding by the lactose repress

A simple and sensitive assay procedure f
A simple and sensitive assay procedure for ribonucleotide reductase system
✍ Yun-Chi Yeh 📂 Article 📅 1978 🏛 Elsevier Science 🌐 English ⚖ 467 KB

A sensitive, rapid, and accurate assay for measuring ribonucleotide reductase activity, including the reduction of CDP. UDP, GDP. and ADP to their corresponding deoxyribonucleotides, is described. Using polyethyleneiminecellulose thin-layer chromatography, I was able to separate the substrates, the