Procedure for uniform binding of DNA to nitrocellulose filters
β Scribed by T.G. Cooper
- Book ID
- 102626464
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 289 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0003-2697
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The ability to bind to nitrocellulose is commonly accepted as being a universal property of proteins and has been widely used in many different fields of study. This property was first exploited in the study of DNAbinding proteins 30 years ago, in studies involving DNA binding by the lactose repress
A routine semiquantitative procedure which permits soluble calcium-binding proteins to be detected following their adsorption to nitrocellulose membrane filters by liquid scintillation counting of specifically bound 45Ca is described. Proteins with high affinity for calcium such as calmodulin and tr