𝔖 Bobbio Scriptorium
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A new spectrophotometric assay for protein in cell extracts

✍ Scribed by Vernon F. Kalb Jr.; Robert W. Bernlohr

Book ID
102982648
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
584 KB
Volume
82
Category
Article
ISSN
0003-2697

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✦ Synopsis

Protein concentrations in cell-free extracts can be determined quickly and accurately using the measured absorbances at 230 and 260 nm. The choice of these two wavelengths, rather than those (280 and 260 nm) used by Warburg and Christian [(1942) Biochem.

Z. 310, 384-4211, decreases interference by nucleic acids and results in sensitivity equivalent to that of the method of Lowry,

πŸ“œ SIMILAR VOLUMES

A simple spectrophotometric assay for fu
A simple spectrophotometric assay for fumarate hydratase in crude tissue extracts
✍ M.D. Hatch πŸ“‚ Article πŸ“… 1978 πŸ› Elsevier Science 🌐 English βš– 333 KB

A procedure is described for assaying fumarate hydratase by coupling malate formed from fumarate to NADP+ reduction via NADP malic enzyme. The procedure is much more sensitive than existing assay methods and circumvents problems particularly associated with the use of these methods for determining f