Direct assay for O6-methylguanine-acceptor protein in cell extracts
โ Scribed by E.A. Waldstein; E.-H. Cao; R.B. Setlow
- Book ID
- 102628384
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 440 KB
- Volume
- 126
- Category
- Article
- ISSN
- 0003-2697
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Protein concentrations in cell-free extracts can be determined quickly and accurately using the measured absorbances at 230 and 260 nm. The choice of these two wavelengths, rather than those (280 and 260 nm) used by Warburg and Christian [(1942) Biochem. Z. 310, 384-4211, decreases interference by
Double-stranded oligonucleotides, 40 bases in length containing an O6-methylguanine in a BamHI restriction site, were developed as substrates for the determination of human O6-alkylguanine-DNA-alkyltransferase (AGT). The assay proved highly sensitive and quantitative. After incubation of the 5'-end-