A new enzymatic assay for aspartate aminotransferase is presented. The 2-oxoglutarate formed in transamination between L-glutamate and oxalacetate was determined in a system coupled with hydroxyglutarate dehydrogenase and NADH by following a decrease in absorbance at 340 nm. The method allowed accur
A new radioactive assay procedure of aromatic aminotransferase
โ Scribed by Sabit Gabay; Harvey George
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 423 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
The assays of aromatic aminotransferase activity can be divided into two broad categories: spectrophotometric and manometric. The spectrophotometric methods are based on either the enol-borate complex formation as originally described by Knox and Pitt (1)) or the heptamolybdatearomatic pyruvate color formation assay of Briggs (2), as modified by Canellakis and Cohen (3). Although these procedures are sensitive and widely used, they possess certain inherent limitations.
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