๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A new assay for l-aspartate: 2-oxoglutarate aminotransferase

โœ Scribed by Toshiharu Yagi; Hiroyuki Kagamiyama; Shigeki Ohtawara; Kenji Soda; Mitsuhiro Nozaki

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
380 KB
Volume
100
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

A new enzymatic assay for aspartate aminotransferase is presented. The 2-oxoglutarate formed in transamination between L-glutamate and oxalacetate was determined in a system coupled with hydroxyglutarate dehydrogenase and NADH by following a decrease in absorbance at 340 nm. The method allowed accurate determination of the initial velocity of the reaction, which was proportional to the enzyme concentration. The Michaelis constants of pig heart cytosolic aspartate aminotransferase for L-glutamate and oxalacetate and the amino acceptor specificity using L-glutamate as an amino donor were determined. The method was applicable to the determination of the enzyme activity in various materials including rat serum -and bacterial crude extract.

๐Ÿ“œ SIMILAR VOLUMES

Electrophoretic and kinetic characteriza
Electrophoretic and kinetic characterization of two phenotypes of soluble cytoplasmic L-aspartate:2-oxoglutarate aminotransferase in human liver tissues
โœ Dr. Fusae Kanemitsu ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 346 KB

## Electrophoretic and kinetic characterization of two phenotypes of soluble cytoplasmic ~-aspartate:2-oxoglutarate aminotransferase in human liver tissues Two phenotypes of soluble cytoplasmic ~-aspartate:2-oxoglutarate aminotransferase (AST, EC 2.6.1. l), sAST 1 and sAST 2-I , were partially puri

A Continuous Spectrophotometric Assay fo
A Continuous Spectrophotometric Assay for Aspartate Transcarbamylase and ATPases
โœ Colin E. Rieger; John Lee; Joanne L. Turnbull ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 162 KB

cause no continuous assay for the detection of free phos-A new continuous coupled uv-spectrophotometric phate had been developed that is sensitive yet also conassay is described for two phosphate-releasing envenient and cost effective. Of the continuous assays zymes, aspartate transcarbamylase and A

A homogeneous assay system of aspartate
A homogeneous assay system of aspartate aminotransferase iso-enzymes using proteases and application for clinical evaluation of myocardial infarction
โœ Koji Yoneda; Yosiaki Katayama; Toshiyo Koike; Isao Tanimizu ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 488 KB

We designed a rapid, homogeneous assay for human aspartate aminotransferase (AST) isoenzymes, by a selective proteolysis of soluble AST (s-AST), using chymotrypsin and protease 401. The linearity of mitochondrial (m-AST) was elongated up to 4000 U/I. m-AST values from the human liver, and determined