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A new method for the detection and assay of α-1,3-glucanases

✍ Scribed by Burton L. Lamberts; Lloyd G. Simonson; Ernest D. Pederson; Robert W. Gaugler

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
633 KB
Volume
117
Category
Article
ISSN
0003-2697

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✦ Synopsis

A calorimetric method that is specific for the assay of a-1,3-glucanases is presented. The enzyme substrate consists of Cibacron Blue F3GA complexed with a dextranase-treated streptococcal glucan. The method is especially convenient for tests involving large numbers of samples, and can be adapted to quantitative as well as qualitative applications. The assay is sufficiently sensitive for screening bacterial samples as potential sources of a-1,3-glucanase.

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