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A New Method for Protein Coexpression in Escherichia coli Using Two Incompatible Plasmids

✍ Scribed by Wei Yang; Lan Zhang; Zhigang Lu; Wei Tao; Zhonghe Zhai

Book ID
115645866
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
145 KB
Volume
22
Category
Article
ISSN
1046-5928

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Amplification of the lactose carrier pro
Amplification of the lactose carrier protein in Escherichia coli using a plasmid vector
✍ Teather, Ron M. ;MΓΌller-Hill, Benno ;Abrutsch, Ursula ;Aichele, Gabriele ;Overat πŸ“‚ Article πŸ“… 1978 πŸ› Springer 🌐 English βš– 932 KB

The isolation and properties of a hybrid plasmid carrying the Y gene of the lac operon of Escherichia coli are described. The lactose carrier protein, coded for by the Y gene, is readily identified upon lac operon induction in strains carrying the plasmid. The protein comprises about 15% of the cyto