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A new logic for DNA engineering using recombination in Escherichia coli

✍ Scribed by Zhang, Youming; Buchholz, Frank; Muyrers, Joep P.P.; Stewart, A. Francis


Book ID
109685100
Publisher
Nature Publishing Group
Year
1998
Tongue
English
Weight
230 KB
Volume
20
Category
Article
ISSN
1061-4036

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A molecular model for conjugational reco
✍ Lloyd, Robert G. ;Thomas, Allyn πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 965 KB

Conjugational recombination in Escherichia coli was investigated by measuring lacZ+ product, beta-galactosidase, in crosses between lacZ mutants. Enzyme production in both Hfr and F-prime crosses was detected very soon after transfer of the donor lacZ allele. The level of enzyme activity was reduced