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A New Vector for Cloning Large Eukaryotic DNA Segments in Escherichia coli

✍ Scribed by Leonardo, Eduardo D. ;Sedivy, John M.

Book ID
109907681
Publisher
Nature Publishing Group
Year
1990
Tongue
English
Weight
801 KB
Volume
8
Category
Article
ISSN
1087-0156

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The in vitro transfer of DNA, was mainly developed by using bacteria as host cells (Bernard and Helinski 1980). Accordingly, suitable vector systems for recombinant DNA technology are predominantly of prokaryotic origin. Since gene cloning is becoming a more essential component of both fundamental r