We have previously shown that DNA gyrase of Escherichia coli can promote recombination between heterologous DNAs in a cell-free system (Ikeda et al. 1982). In the present paper, we have studied the nucleotide sequences of several recombination junctions of lambda-pBR322 recombinants and found that t
DNA cloning by homologous recombination in Escherichia coli
β Scribed by Stewart, A. Francis; Zhang, Youming; Muyrers, Joep P.P.; Testa, Giuseppe
- Book ID
- 109829570
- Publisher
- Nature Publishing Group
- Year
- 2000
- Tongue
- English
- Weight
- 106 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1087-0156
- DOI
- 10.1038/82449
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DNA fragments of phage PM2 restricted with Hin dIII endonuclease was cloned in the vector pBR 322 in an Escherichia coli K12 host. The attempt to clone full length PM2 DNA restricted with PstI endonuclease has been unsuccesful. From six randomly chosen recombinant clones DNA was purified and analyse