𝔖 Bobbio Scriptorium
✦   LIBER   ✦

DNA cloning by homologous recombination in Escherichia coli

✍ Scribed by Stewart, A. Francis; Zhang, Youming; Muyrers, Joep P.P.; Testa, Giuseppe


Book ID
109829570
Publisher
Nature Publishing Group
Year
2000
Tongue
English
Weight
106 KB
Volume
18
Category
Article
ISSN
1087-0156

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Homology is not required for recombinati
✍ Naito, Avako ;Naito, Satoshi ;Ikeda, Hideo πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 688 KB

We have previously shown that DNA gyrase of Escherichia coli can promote recombination between heterologous DNAs in a cell-free system (Ikeda et al. 1982). In the present paper, we have studied the nucleotide sequences of several recombination junctions of lambda-pBR322 recombinants and found that t

Homologous recombination promoted by Chi
✍ Gerald R. Smith; Franklin W. Stahl πŸ“‚ Article πŸ“… 1985 πŸ› John Wiley and Sons 🌐 English βš– 873 KB

Chi sites are examples of special sites enhancing homologous recombination in their region of the chromosome. Chi, SG-C-T-G-G-T-G-GY, is a recognition site for the RecBC enzyme, which nicks DNA near Chi as it unwinds DNA. A molecular model ofgenetic recombination incorporating these features is revi

Cloning of bacteriophage PM2 DNA in Esch
✍ Rempola, BoΕΌena ;Fikus, Magdalena πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 964 KB

DNA fragments of phage PM2 restricted with Hin dIII endonuclease was cloned in the vector pBR 322 in an Escherichia coli K12 host. The attempt to clone full length PM2 DNA restricted with PstI endonuclease has been unsuccesful. From six randomly chosen recombinant clones DNA was purified and analyse

Gene Recombination in Escherichia Coli
✍ LEDERBERG, JOSHUA; TATUM, E. L. πŸ“‚ Article πŸ“… 1946 πŸ› Nature Publishing Group 🌐 English βš– 161 KB