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Cloning of bacteriophage PM2 DNA in Escherichia coli K12

✍ Scribed by Rempola, Bożena ;Fikus, Magdalena

Publisher
Springer
Year
1979
Tongue
English
Weight
964 KB
Volume
176
Category
Article
ISSN
0026-8925

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✦ Synopsis

DNA fragments of phage PM2 restricted with Hin dIII endonuclease was cloned in the vector pBR 322 in an Escherichia coli K12 host. The attempt to clone full length PM2 DNA restricted with PstI endonuclease has been unsuccesful. From six randomly chosen recombinant clones DNA was purified and analysed with EcoRI, PstI and Hin dIII endonucleases. The physical map of three chimeric plasmids was unequivocally established. It was shown, that the whole PM2 genome was cloned, although in separate fragments. However, most of the recombinant clones were instable in the absence of selective pressure.

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