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A new method for determining anti-B cell antibodies and their specificity using flow cytometry

โœ Scribed by A.J. Berteli; V. Daniel; P. Terness; G. Opelz


Book ID
118902708
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
394 KB
Volume
164
Category
Article
ISSN
0022-1759

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โœ Katsumata, Y. ;Sato, M. ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 209 KB

A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human erythrocytes is described. Specific antibodies were first absorbed with the corresponding cells, then eluted by heating at 53ยฐ-55ยฐC for 15 min. The method is simple and highly efficient with a fair reco