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A new detection method for arginine-specific ADP-ribosylation of protein — A combinational use of anti-ADP-ribosylarginine antibody and ADP-ribosylarginine hydrolase

✍ Scribed by Harumi Osago; Masaharu Terashima; Nobumasa Hara; Kazuo Yamada; Mikako Tsuchiya

Book ID: 118479072
Publisher: Elsevier Science
Year: 2008
Tongue: English
Weight: 588 KB
Volume: 70
Category: Article
ISSN: 0165-022X
DOI: 10.1016/j.jprot.2007.11.008

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Detection of Arginine-ADP-Ribosylated Pr

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✍ T. Ohno; M. Tsuchiya; H. Osago; N. Hara; J. Jidoi; M. Shimoyama 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 788 KB

We made use of ADP-ribosylarginine hydrolase to detect arginine-ADP- ribosylated proteins. The hydrolase was expressed in Escherichia coli as a protein fused with glutathione S-transferase (GST). The fusion protein GST-ADP-ribosylarginine hydrolase catalyzed the hydrolysis of alpha-ADP-ribosylargini