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A new method for allantoin determination and its application in allantoin determination in Agrostemma githago L. seed

✍ Scribed by Miladin M. Vrbaški; Bojana Grujić-Injac; Danica Gajić

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
248 KB
Volume
91
Category
Article
ISSN
0003-2697

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✦ Synopsis

We have established several optimal conditions for qualitative and quantitative allantoin determination by applying Ehrlich's reagent. The limit of detection for allantoin determination amounts to 5 x 10(-6) mM. Allantoin is determined quantitatively by measuring the absorbance at 440 nm (from 300 to 1000 micrograms/ml). The color of the complex becomes stable by standing for 10 min at room temperature. We have used these conditions for allantoin determination in Agrostemma githago seed.

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