A new individualization marker of semen: Deoxyribonuclease I (DNase I) polymorphism

✍ Scribed by Kazumi Sawazaki; Toshihiro Yasuda; Daita Nadano; Etsuko Tenjo; Reiko Iida; Haruo Takeshita; Koichiro Kishi

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 928 KB
• Volume: 57
• Category: Article
• ISSN: 0379-0738
• DOI: 10.1016/0379-0738(92)90043-v

✦ Synopsis

We describe a method for obtaining specific and reproducible deoxyribonuclease I (DNase I) typing from liquid semen. Isoelectric focusing of the enzymes on polyacrylamide gel (IEF-PAGE, pH 3.5-5) was accomplished using a 0.5-mm thick gel. The separated isozymes were visualized by a new activity staining method, dried agarose film-overlay (DAFO). Pretreatment of semen samples with neuraminidase markedly enhanced the isozyme-band resolution and sensitivity. The method was simple and reliable, with high resolution and sensitivity. The DNase I types in semen samples were correlated with the types found in corresponding blood and urine samples. DNase I typing could therefore provide an additional discriminant characteristic in the forensic examination of semen.