We describe a method for obtaining specific and reproducible deoxyribonuclease I (DNase I) typing from liquid semen. Isoelectric focusing of the enzymes on polyacrylamide gel (IEF-PAGE, pH 3.5-5) was accomplished using a 0.5-mm thick gel. The separated isozymes were visualized by a new activity stai
The fifth allele of the human deoxyribonuclease I (DNase I) polymorphism
β Scribed by Reiko Iida; Toshihiro Yasuda; Masahiro Aoyama; Etsuko Tsubota; Michiko Kobayashi; Isao Yuasa; Takasumi Matsuki; Koichiro Kishi
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 381 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0173-0835
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This study confirms the presence of a novel variable number of tandem repeats polymorphism, designated as HumDN1, in intron 4 of the human deoxyribonuclease I (DNase I) gene. Genotyping was performed without difficulty by PCR-amplification and separation by agarose gel electrophoresis in 423 Japanes
## Abstract Members of the human DNase I family, DNase Iβlike 1 and 2 (DNases 1L1 and 1L2), with physiological role(s) other than those of DNase I, possess three and one nonβsynonymous SNPs in the genes, respectively. However, only limited population data are available, and the effect of these SNPs