A new gene for ribosomal restriction inEscherichia coli
β Scribed by Robert T. Garvin; Luigi Gorini
- Book ID
- 104726283
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 365 KB
- Volume
- 137
- Category
- Article
- ISSN
- 1617-4615
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β¦ Synopsis
Two mutations restricting the leakiness of an amber mutant are described. They were selected without the use of streptomycin: one maps in the strA region (at 64 minutes of the current E. coli chromosomal map) but is streptomycin sensitive and the other in the threonine region (at the origin of the map), 23% cotransducible with threonine by P1.
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Two genes coding for endoglucanase activity in Clostridium cellulolyticum were cloned and expressed in Escherichia coli by using plasmid pUC18. The sizes of two fragments harbouring endoglucanase genes are 4.4 kb and 2.0 kb, respectively. The 2.0-kb fragment was identical with a reported D N A fragm