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A method for generating precise gene deletions and insertions inEscherichia coli

โœ Scribed by Qi-Ming Zhou; Dong-Jie Fan; Jiang-Bi Xie; Chuan-Peng Liu; Jun-Mei Zhou


Book ID
106565262
Publisher
Springer
Year
2010
Tongue
English
Weight
311 KB
Volume
26
Category
Article
ISSN
1573-0972

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A polymerase chain reaction (PCR) based procedure for the isolation of genes in transposon or Tโ€DNA tagging approaches has been developed. The method can be generally applied and allows the rapid isolation of putative gene sequences even in the presence of high numbers of insertion sequences. The te