✦ LIBER ✦
A general method for gene isolation in tagging approaches: amplification of insertion mutagenised sites (AIMS)
✍ Scribed by Monika Frey; Cornelia Stettner; Alfons Gierl
- Book ID
- 104463776
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 213 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0960-7412
No coin nor oath required. For personal study only.
✦ Synopsis
A polymerase chain reaction (PCR) based procedure for the isolation of genes in transposon or T‐DNA tagging approaches has been developed. The method can be generally applied and allows the rapid isolation of putative gene sequences even in the presence of high numbers of insertion sequences. The technique has been used successfully for the isolation of the maize Bx1 gene tagged by a Mutator element.