✦ LIBER ✦

A new fluorometric method for determination of picomoles of inorganic phosphorus. Application to the renal tubular fluid

✍ Scribed by Michele G. Brunette; Normand Vigneault; Gaby Danan

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 422 KB
Volume: 86
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90338-x

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✦ Synopsis

A new method for the determination of inorganic phosphorus (Pi) in 4 nl of renal tubular fluid is described. Phosphate is converted to hexadimolybdatophosphate. which is reacted with thiamine to produce a highly fluorescent thiochrome. The reaction is stable and reproducible. Problems of interference are minimal. Microdeterminations of Pi in the proximal tubules and glomerular filtrates of the rat yielded results similar to those published in the literature.

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