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Fluorometric method for the determination of magnesium in renal tubular fluid

✍ Scribed by Michele G. Brunette; M.E. Crochet

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
487 KB
Volume
65
Category
Article
ISSN
0003-2697

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✦ Synopsis

The fluorescent reaction between the N,N'-bis-salicylidene 2,3, diamino benzofuran (SABF) and magnesium (Mg) can be utilized for determination of Mg in nanoliter volumes of renal tubular fluid. Problems of interference are minimal and have been overcome by adding inorganic phosphorus to the standards. Mg in urine may also be determined by this technique if urines of known Mg content are utilized as standards, and if both samples and standards are diluted l/IO in artificial tubular fluid. Microdetermination of Mg in glomerular filtrates of Munich Wistar rats confirms that the UF Mg is isotonically filtered through the glomerular membrane.

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