A new fluorescence standard
β Scribed by Hanna Bulska
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 559 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0022-2313
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The absorption and fluorescence spectra, fluorescence yields and decay times of all trans-1,6\_diphenyI-1,3,5hexatriene @PHI have been measured over a range of temperature in several different solvents. In non-polar solvents fluorescence yields decrease with increase of temperature whilst decay time
The growing number of standards commercially available in the field of flow cytometry makes it difficult to know which standards to use to obtain a desired level of quality assurance. A classification system of fluorescence standards has been developed on the basis of their physical characteristics.
Background: Numerous applications of fluorescence microscopy require quantitation of signal intensity in reproducible units. Two problems must be overcome to achieve this goal. First, due to various instrumental factors, the same sample imaged on two microscopes or even on the same microscope at dif