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A new chemical method of synthesis of modified nucleoside [32P]phosphates

✍ Scribed by D. V. Yanvarev; E. A. Shirokova; M. K. Kukhanova; Yu. S. Skoblov

Publisher
John Wiley and Sons
Year
2008
Tongue
French
Weight
131 KB
Volume
51
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

A simple method of chemical phosphorylation for modified nucleosides with [^32^P]orthophosphoric acid in the presence of BrCN is described. The yields of 5′‐[^32^P]nucleoside monophosphates achieved are 50–65% at nominal specific radioactivities of ca. 1000 Ci/mmol. The mechanism of phosphorylation in the presence of heterocyclic amines is studied. Copyright Β© 2008 John Wiley & Sons, Ltd.

πŸ“œ SIMILAR VOLUMES

A simple procedure for the synthesis of
A simple procedure for the synthesis of Ξ±-32P-nucleoside triphosphates
✍ Christof K. Biebricher πŸ“‚ Article πŸ“… 1979 πŸ› Elsevier Science 🌐 English βš– 306 KB

A simple method for preparing s-32P-labeled nucleoside-5'-triphosphates of high specific activity is presented. Unprotected nucleosides are heated dry with urea and inorganic phosphate. The products from such reactions are phosphorylated enzymatically.

Diastereoselective Synthesis of N,O-Psic
Diastereoselective Synthesis of N,O-Psiconucleosides, a New Class of Modified Nucleosides
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Anomeric Ξ±and Ξ²-N,O-psiconucleosides were prepared by 1,3-dipolar cycloaddition of C-ethoxycarbonyl N-methyl nitrone with ethyl 2-acetyloxyacrylate, followed by VorbrΓΌggen nucleosidation. The synthetic scheme has been applied to all purine and pyrimidine nucleobases. Nucleosidation can proceed under