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A new assay of estrogen receptor by thin-layer gel filtration

โœ Scribed by Tadahiko Hazato; Akira Murayama; Akio Matsuzawa; Tadashi Yamamoto

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
517 KB
Volume
94
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Application of a thin-layer gel filtration (TLG) method using Sephadex G-200 superline for the assay of estrogen receptor (ER) is described. The method is based on the effective separation of specific receptor protein from nonspecific binding proteins and free hormone by TLG and subsequent determination of the radioactivity bound to specific ER. By the aid of fluorescein-labeled human -y-globulin, the position of specific ER can be visualized. The assay excludes completely the undesired fluctuation depending on the concentration of coexisting proteins and permits reliable and rapid quantitation of specific ER. Association constant and concentration of specific ER can be determined with 100 mg of mammary tumor tissue. The method has the additional advantage of not requiring expensive equipment.

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