Rapid and efficient separation and identification of the two molecular forms of human adenosine deaminase by thin-layer gel filtration chromatography

✍ Scribed by Pang Fai Ma; Steve Betras; Gary Dunnington

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 904 KB
• Volume: 75
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90068-3

✦ Synopsis

Two molecular forms of adenosine deaminase have been found in human tissues. The column gel filtration method has been used for the separation of the two enzyme forms. Routine separation and analysis of the enzyme forms based on the molecular size difference can be achieved by thin-layer gel filtration on Sephadex G-200 superfine gel. The thin-layer method has been found to be more rapid and efficient than the column method. Enzymes in crude preparations can be studied effectively with the thin-layer method.

MATERIALS

Adenosine was obtained from Calbiochem.

Calf intestinal adenosine deaminase, rabbit muscle aldolase, bovine thyroglobulin, human transfer-tin, y-globulin, and horse heart cytochrome c were purchased from Sigma. Chymotrypsinogen A was obtained from Nutritional Biochemicals Corp., ovalbumin from Schwarz/Mann, and bovine serum albumin 177 Copyright 0 1976 by Academic Press. Inc. All r&t\ of reproduction in any form rexwed.