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A Multiplex PCR Method for the Identification of Commercially Important Salmon and Trout Species (Oncorhynchus and Salmo) in North America

✍ Scribed by Rosalee S. Rasmussen Hellberg; Michael T. Morrissey; Robert H. Hanner


Book ID
111406497
Publisher
Institute of Food Technologists
Year
2010
Tongue
English
Weight
567 KB
Volume
75
Category
Article
ISSN
0022-1147

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A DNA-based method (PCR-RFLP) has been developed for discrimination between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). The polymerase chain reaction (PCR) was used for ampli®cation of a 464 bp fragment of the mitochondrial cytochrome oxidase subunit II (COII) gene. Digest