PCR-RFLP of the mitochondrial cytochrome oxidase gene: a simple method for discrimination between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss)
✍ Scribed by Carrera, Esther; Garc�a, Teresa; C�spedes, Ana; Gonz�lez, Isabel; Fern�ndez, Alicia; Hern�ndez, Pablo E; Mart�n, Rosario
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 193 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
A DNA-based method (PCR-RFLP) has been developed for discrimination between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). The polymerase chain reaction (PCR) was used for ampli®cation of a 464 bp fragment of the mitochondrial cytochrome oxidase subunit II (COII) gene. Digestion of the products with endonucleases Nci I and Sau 3AI, followed by agarose gel electrophoresis of the digested products, yielded speci®c restriction pro®les that enabled direct visual identi®cation of the species analysed. This PCR-RFLP methodology allowed clear discrimination of Atlantic salmon and rainbow trout samples both in raw and smoked products.