✦ LIBER ✦

A modified method for estimation of ornithine in biological samples

✍ Scribed by R. Gopalakrishna; B. Nagarajan

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 294 KB
Volume: 101
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90215-8

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✦ Synopsis

A rapid ion-exchange chromatographic method was developed for the separation of tissue ornithine from other amino acids, except lysine, interfering with Chinard's ninhydrin method. The reaction was modified to estimate ornithine in the presence of lysine by reducing the color development time to 20 min and by benzene extraction of the chromogen formed. Chromogens of omithine and lysine have 5201430 nm absorbance ratios of 4 and 1.3, respectively, and this was exploited to correct for lysine interference.

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