A gas chromatographic method for the analysis of lipoic acid in biological samples

✍ Scribed by Robert H. White

• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 325 KB
• Volume: 110
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90116-0

✦ Synopsis

A method has been developed for the gas chromatographic analysis of lipoic acid in biological samples. The lipoic acid is released from the samples by acid hydrolysis in the presence of the internal standatds 1,2-dithiolane-3-butyric acid and/or 1,2-dithiolane-3caproic acid. After hydrolysis, the lipoic acid and the internal standards are extracted from the hydrolysate and converted into the S,Sdibenzylmethyl esters. Gas chromatographic analysis of this mixture completely separates each of the homolog derivatives from the lipoic acid derivative and allows for the quantitation of the lipoic acid in the sample. Samples containing more than -50 ng of lipoic acid can be easily assayed. Results are presented that show that the lipoic acid content of Escherichia cofi depends on the carbon source used for its growth.