Estimation of monosaccharide radioactivity in biological samples through osazone derivatization
✍ Scribed by F.J. García; A. Pons; M. Alemany; A. Palou
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 423 KB
- Volume
- 120
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A method for the quantitative estimation of radioactivity in the glucose (monosaccharide) fraction of biological samples is presented. Radioactive samples are added with cold glucose, and 1 aliquot receives a known amount of radioactive glucose as internal standard. After controlled osazone formation and three washings of the yellow precipitate, the osazones are dissolved, decolored, and their radioactivity determined through scintillation counting. The overall efficiency of recovery is 23-24% of the initial radioactivity. Each sample is corrected by the recovery of its own internal standard. There is a very close linear relationship between radioactivity present in the samples and radioactivity found, despite the use of different biological samples (rat plasma, hen egg yolk and albumen).