✦ LIBER ✦

A modified equilibrium dialysis method for studying fatty acid binding to proteins

✍ Scribed by R.A. Klein; H.J.K. Keuper

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 492 KB
Volume: 30
Category: Article
ISSN: 0009-3084
DOI: 10.1016/0009-3084(82)90008-1

No coin nor oath required. For personal study only.

✦ Synopsis

A modified equilibrium dialysis method is described which is suitable for investigating the binding of fatty acids in the form of aqueous micellar dispersions to proteins. The method uses a permeant chromophore which complexes reversibly with free fatty acid within the dialysis bag. The concentration outside the dialysis bag is determined spectrophotometrically. Binding of oleic acid to bovine serum albumin is given as an example. A simplified analysis of fatty acid binding is given and used to indicate the potential of the method.

📜 SIMILAR VOLUMES

The analysis of fatty acid binding to pr

The analysis of fatty acid binding to protein using a modified equilibrium dialysis method: detailed analysis of chromophore—fatty acid—protein interactions

✍ H.J.K. Keuper; R.A. Klein; F. Spener 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 496 KB

In this paper we extend our previous analysis of fatty acid-chromophore-protein interactions using a modified equilibrium dialysis method described previously. A more rigorous mathematical treatment is combined with a micro-dialysis method using a maximum volume of dialyzate of between 250 gl and 40

Determination of equilibrium constants a

Determination of equilibrium constants and binding capacities using a modified scatchard method in drug-protein binding studies

✍ Joseph K. H. Ma; H. W. Jun; L. A. Luzzi 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 English ⚖ 285 KB 👁 1 views

Dynamic dialysis as a method for studyin

Dynamic dialysis as a method for studying protein binding II: Evaluation of the method with a number of binding systems

✍ Marvin C. Meyer; David E. Guttman 📂 Article 📅 1970 🏛 John Wiley and Sons 🌐 English ⚖ 981 KB

Covalent Binding of Modified Nucleic Aci

Covalent Binding of Modified Nucleic Acids to Proteins as a Method for Investigation of Specific Protein—Nucleic Acid Interactions

✍ T. S. Zatsepin; N. G. Dolinnaya; E. A. Kubareva; M. G. Ivanovskaya; V. G. Metele 📂 Article 📅 2005 🏛 John Wiley and Sons ⚖ 8 KB 👁 2 views

Dynamic dialysis as a method for studyin

Dynamic dialysis as a method for studying protein binding I: Factors affecting the kinetics of dialysis through a cellophane membrane

✍ Marvin C. Meyer; David E. Guttman 📂 Article 📅 1970 🏛 John Wiley and Sons 🌐 English ⚖ 721 KB

Rapid methods for the characterization o

Rapid methods for the characterization of unilamellar phospholipid vesicles. Application to studies on fatty acid donor and acceptor properties of membranes and fatty acid binding proteins

✍ M. Reers; R. Elbracht; H. Rüdel; F. Spener 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 615 KB

Vesicles having diameters from 20 to 200 nm were prepared from egg-yolk phosphatidylcholine (PC) and were separated as well as analyzed by methods that can be carried out with standard laboratory equipment. Gel-chromatography on Sephacryl S 1000 was adapted for expeditious size analysis of vesicles