A modified assay of 3′:5′-cyclic-AMP phosphodiesterase
✍ Scribed by R.J. Boudreau; G.I. Drummond
- Book ID
- 107711705
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 705 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0003-2697
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A new method for assay of cyclic AMP phosphodiesterase (EC 3.1.4.17) has been developed based on the observation that a mixture of cyclic AMP and AMP can be resolved on a column of florisil (activated magnesium silicate) at pH 7.0. The cyclic nucleotide is retained by the silicate and the AMP which
In the course of studies on cyclic nucleotide phosphodiesterase, the need for an assay which was both sensitive and continuous was realized. Most of the methods available for monitoring phosphodiesterase either depend on the use of accessory enzymes, and are accordingly subject to intrinsic limitati