A modified assay for cholinesterase
β Scribed by D.B. Hoover; J. Kosa; B.K. Colasanti; C.R. Craig
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 228 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0026-265X
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π SIMILAR VOLUMES
A semimicrotitration device for reaction volumes of 0.5 to 2 ml and effective stirring of reaction solution or suspension is described. The principal part of the device is a combined glass electrode functioning as a reaction vessel (combined "cup"-glass electrode). Characteristics of the device are
A rapid and simple radiometric assay for cholinesterase, suitable for multiple determinations, has been developed. (aH-acetyl) choline is enzymatically hydrolyzed in a small reaction volume in a scintillation vial. The released [all]acetate is then extracted into a toluene-based scintillator added d
## Cholincstcrase catalyses the hydrolysis of acetylcholine which acts at nerve endings ;is 11 transmitter of impulses from nerve to muscle fiber'. Among several analytical procedures which huvc been developed for the assay of cholincstcrase. lluorimctric and spcctrophotometric methods are very fr