A model for micro/ultrafiltration cell deactivation in cell-recycle reactors

✍ Scribed by João A P Coutinho; Ana M R B Xavier

• Publisher: Wiley (John Wiley & Sons)
• Year: 2000
• Tongue: English
• Weight: 110 KB
• Volume: 75
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/(sici)1097-4660(200004)75:4<315::aid-jctb219>3.0.co;2-p

✦ Synopsis

Mechanical stress due to micro/ultra®ltration has been suggested by a number of authors as one of the sources for cell deactivation in continuous cell-recycle reactors. This work introduces cell deactivation in the modelling of cell-recycle fermenters. A two-population based model is used. It is shown that the kinetic parameters obtained from chemostat fermentations describe accurately the experimental results and the apparent deviations to the Luedeking±Piret relationship at low growth rates are explained. The model is applied to the data of two authors with dilution rates (D) ranging 0.2 and 1.05 h À1 and using either Lactobacillus rhamnosus or Lactococcus cremoris cells. Although only data for lactic fermentation were used, the proposed model is of general applicability and not restricted to a type of fermentation, a given apparatus con®guration or Newtonian ¯uids. The development of improved procedures for biological reactor modelling, such as presented in this work, wall allow more adequate and ef®cient design and operation of these reactors.