Murine teratocarcinoma: A model for virus-cell interaction in a differentiating cell system

✍ Scribed by Friedrich, Thomas D. ;Lehman, John M.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1981
• Tongue: English
• Weight: 479 KB
• Volume: 15
• Category: Article
• ISSN: 0275-3723
• DOI: 10.1002/jsscb.1981.380150211

✦ Synopsis

Abstract

The stem cell of the murine teratocarcinoma is refractory to infection with Simian virus 40 and polyoma. Utilizing various procedures, we attempted to alter this block to infection by modifying the infection procedure. Multiple infections with high‐titer SV~40~ and pretreatment of cells with DEAE‐dextran or the carcinogen 4‐nitroquinoline l‐oxide did not induce embryonal carcinoma cells to produce T‐ antigen. Co‐infection with adenovirus 5, which infects the embryonal carcinoma, and SV~40~ did not induce the expression of SV~40~ Tantigen. Therefore, these procedures did not overcome the block to virus infection. The assay for the SV~40~ T antigen was immunofluorescence; however, the immunoprecipitation technique did not detect T antigen in the infected embryonal carcinoma cells. Finally, the viral DNA present in the embryonal carcinoma was examined for its ability to replicate. These studies showed that viral DNA was not replicating as assayed by the viral DNA's sensitivity to UV irradiation when replicating in the presence of 5‐bromodeoxyundine.