𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction

✍ Scribed by S. Fronhoffs; G. Totzke; S. Stier; N. Wernert; M. Rothe; T. Brüning; B. Koch; A. Sachinidis; H. Vetter; Y. Ko

Book ID
115638475
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
316 KB
Volume
16
Category
Article
ISSN
0890-8508

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

A New Quantitative Method of Real Time R
A New Quantitative Method of Real Time Reverse Transcription Polymerase Chain Reaction Assay Based on Simulation of Polymerase Chain Reaction Kinetics
✍ Weihong Liu; David A. Saint 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 108 KB

Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference

Quantitation of Cytosine Deaminase mRNA
Quantitation of Cytosine Deaminase mRNA by Real-Time Reverse Transcription Polymerase Chain Reaction: A Sensitive Method for Assessing 5-Fluorocytosine Toxicity in Vitro
✍ C.Ryan Miller; Allen N. Gustin; Donald J. Buchsbaum; Selwyn M. Vickers; Upender 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 451 KB

Cytosine deaminase/5-fluorocytosine (CD/5-FC) is a promising strategy for local cancer gene therapy. We hypothesized that CD expression within tumor cells would be directly related to efficacy and that quantitation of markers of CD expression such as mRNA, protein, and enzyme activity would therefor