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Establishment and Application of a TaqMan Real-Time Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Rubella Virus RNA

✍ Scribed by Li-Hong ZHAO; Yu-Yan MA; Hong WANG; Shu-Ping ZHAO; Wei-Ming ZHAO; Hua LI; Lei-Yi WANG

Book ID
111357715
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
677 KB
Volume
38
Category
Article
ISSN
1672-9145

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A New Quantitative Method of Real Time R
A New Quantitative Method of Real Time Reverse Transcription Polymerase Chain Reaction Assay Based on Simulation of Polymerase Chain Reaction Kinetics
✍ Weihong Liu; David A. Saint πŸ“‚ Article πŸ“… 2002 πŸ› Elsevier Science 🌐 English βš– 108 KB

Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference