✦ LIBER ✦

A method for the chemical synthesis of 14C-labeled fatty acyl coenzyme A's of high specific activity

✍ Scribed by James E. Bishop; Amiya K. Hajra

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 537 KB
Volume: 106
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90531-x

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✦ Synopsis

A simple and reliable method, based on that described by W. Seubert (1960, Biochem Prep. 7, 80-83), has been developed for the chemical synthesis of radioactive acyl coenzyme A's. I-14C-labeled fatty acids (palmitic, oleic, and linoleic) are converted to their acyl chlorides with oxalyl chloride. The [l-14C]acyl chlorides are then condensed with a two-to three-fold molar excess of coenzyme A in a bicarbonate-buffered tetrahydrofuran solution to form the corresponding [l-14C]acyl coenzyme A's. The overall yields are near 75%, and the purities are greater than 90% based on spectral, chromatographic, and enzymatic properties.

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